Whole-genome sequencing as a tool for studying the microevolution of drug-resistant serial Mycobacterium tuberculosis isolates

ABSTRACT Treatment of drug-resistant tuberculosis requires extended use of more toxic and less effective drugs and may result in retreatment cases due to failure, abandonment or disease recurrence. It is therefore important to understand the evolutionary process of drug resistance in Mycobacterium tuberculosis. We here in describe the microevolution of drug resistance in serial isolates from six previously […]
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In silico and in vitro evaluation of tetrahydropyridine compounds as efflux inhibitors in Mycobacterium abscessus

Authors: Ramis IB, Vianna JS, Silva Junior L, von Groll A, Ramos DF, Zanatta N, Viveiros M, Silva PEAD
Publication Year: 2019
Journal: Tuberculosis
Link: https://www.sciencedirect.com/science/article/pii/S1472979219300654

Herein, we evaluated tetrahydropyridine (THP) compounds (NUNM) as antimicrobials and inhibitors of the efflux mechanism in M. abscessus. subsp. abscessus. The modulation factor (MF) of efflux inhibitors was calculated from the minimum inhibitory concentrations (MICs) of amikacin (AMI), ciprofloxacin (CIP) and clarithromycin (CLA) in the absence and presence of subinhibitory concentrations of the NUNM compounds and canonical […]
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The EU approved antimalarial pyronaridine shows antitubercular activity and synergy with rifampicin, targeting RNA polymerase

Authors: Mori G, Orena BS, Franch C, Mitchenall LA, Godbole AA, Rodrigues L, Aguilar-Pérez C, Zemanová J, Huszár S, Forbak M, Lane TR, Sabbah M, Deboosère N, Frita R, Vandeputte A, Hoffmann E, Russo R, Connell N, Veilleux C, Kumar R, Kumar P, Freundlich JS, Brodin P, Ainsa JA, Nagaraja V, Maxwell A, Mikušová K, Pasca MR, Ekins S
Publication Year: 2018
Journal: Tuberculosis
Link: https://www.tuberculosisjournal.com/article/S1472-9792(18)30008-8/pdf

The search for compounds with biological activity for many diseases is turning increasingly to drug repurposing. In this study, we have focused on the European Union-approved antimalarial pyronaridine which was found to have in vitro activity against Mycobacterium tuberculosis (MIC 5 μg/mL). In macromolecular synthesis assays, pyronaridine resulted in a severe decrease in incorporation of 14C-uracil […]
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Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis

Authors: Baptista PV, Couto I, Machado D, Pedrosa P, Veigas B, Viveiros M
Publication Year: 2014
Journal: Tuberculosis
Link: http://www.sciencedirect.com/science/article/pii/S147297921400002X

Here we extend the Au-nanoprobe approach towards discriminating specific mutations within inhA and rpoB genes in PCR amplified DNA from isolates. Using a multiplex PCR reaction for these two genes, it is possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDRTB molecular characterization with special application in the most frequent Portuguese genotypes.
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Whole-genome sequencing as a tool for studying the microevolution of drug-resistant serial Mycobacterium tuberculosis isolates

In silico and in vitro evaluation of tetrahydropyridine compounds as efflux inhibitors in Mycobacterium abscessus

The EU approved antimalarial pyronaridine shows antitubercular activity and synergy with rifampicin, targeting RNA polymerase

Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis

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