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Home / Archives for Baptista PV

Gold on paper-paper platform for Au-nanoprobe TB detection.

  • Autores: Baptista PV, Barquinha P, Costa MN, Fortunato E, Inácio J, Jacob JM, Martins R, Santos DS, Veigas B, Viveiros M
  • Journal: Lab on a Chip
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Gold+on+paper-paper+platform+for+Au-nanoprobe+TB+detection

Tuberculosis (TB) remains one of the most serious infectious diseases in the world and the rate of new cases continues to increase. The development of cheap and simple methodologies capable of identifying TB causing agents belonging to the Mycobacterium tuberculosis Complex (MTBC), at point-of-need, in particular in resource-poor countries where the main TB epidemics are observed, is of paramount relevance for the timely and effective diagnosis and management of patients.
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Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis

  • Autores: Baptista PV, Couto I, Machado D, Pedrosa P, Veigas B, Viveiros M
  • Ano de Publicação: 2014
  • Journal: Tuberculosis
  • Link: http://www.sciencedirect.com/science/article/pii/S147297921400002X

Here we extend the Au-nanoprobe approach towards discriminating specific mutations within inhA and rpoB genes in PCR amplified DNA from isolates. Using a multiplex PCR reaction for these two genes, it is possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDRTB molecular characterization with special application in the most frequent Portuguese genotypes.
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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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