Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Authors: Phelan J, De Sessions PF, Tientcheu L, Perdigão J, Machado D, Hasan R, Hasan Z, Bergval I, Anthony R, McNerney R, Antonio M, Portugal I, Viveiros M, Campino S, Hibberd ML, Clark TG
Publication Year: 2018
Journal: Scientific Reports
Link: https://www.nature.com/articles/s41598-017-18188-y%20

DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosis complex is poorly understood but may be involved in virulence, hypoxic survival and the emergence of […]
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Clonal expansion across the seas as seen through CPLP-TB database: A joint effort in cataloguing Mycobacterium tuberculosis genetic diversity in Portuguese-speaking countries

Authors: Perdigão J, Silva C, Diniz J, Pereira C, Machado D, Ramos J, Silva H, Abilleira F, Brum C, Reis AJ, Macedo M, Scaini JL, Silva AB, Esteves L, Macedo R, Maltez F, Clemente S, Coelho E, Viegas S, Rabna P, Rodrigues A, Taveira N, Jordao L, Kritski A, Lapa e Silva JR, Mokrousov I, Couvin D, Rastogi N, Couto I, Pain A, McNerney R, Clark TG, von Groll A, Dalla Costa ER, Rossetti ML, Silva PEA, Viveiros M, Portugal I
Publication Year: 2019
Journal: Infection Genetics and Evolution
Link: https://www.sciencedirect.com/science/article/pii/S1567134818301023?via%3Dihub%20

Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. To address […]
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Genomic diversity of drug-resistant Mycobacterium tuberculosis isolates in Lisbon Portugal: Towards tuberculosis genomic epidemiology

Authors: Clark T, Coll F, Couto I, Hill-Cawthorne GA, Jordao L, Macedo R, Machado D, Mallard K, Maltez F, McNerney R, Pain A, Perdigão J, Portugal I, Silva C, Silva H, Viveiros M
Publication Year: 2015
Journal: International Journal of Mycobacteriology
Link: https://www.sciencedirect.com/science/article/pii/S2212553115000254

Multidrug- (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) present a challenge to disease control and elimination goals. Lisbon, Portugal, has a high TB incidence rate and unusual and successful XDR-TB strains that have been found in circulation for almost two decades. For the last 20 years, a continued circulation of two phylogenetic clades, Lisboa3 and […]
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Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa

Authors: Armada A, Colombatti R, Gomes VF, Machado D, Mane M, Martins E, Ponce G, Rabna P, Ramos J, Sanca L, Vieira F
Publication Year: 2015
Journal: PLoS One
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127536%20

This study aimed to evaluate the usefulness of the Xpert MTB/RIF assay for the rapid direct detection of M. tuberculosis complex (MTBC) strains and rifampicin resistance associated mutations in a resource-limited setting such as Guinea-Bissau and its implications in the management of tuberculosis (TB) and drug resistant tuberculosis, complementing the scarce information on resistance and […]
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Boosting Effect of 2-Phenylquinoline Efflux Inhibitors in Combination with Macrolides against Mycobacterium smegmatis and Mycobacterium avium

Authors: Cannalire R, Cecchetti V, Couto I, Machado D, Manfroni G, Sabatini S, Santos Costa S, Tabarrini O, Viveiros M
Publication Year: 2015
Journal: ACS Infectious Diseases
Link: https://pubs.acs.org/doi/full/10.1021/acsinfecdis.5b00052

The identification of efflux inhibitors to be used as adjuvants alongside existing drug regimens could have a tremendous value in the treatment of any mycobacterial infection. Here, we investigated the ability of four 2-(4′-propoxyphenyl)quinoline Staphylococcus aureus NorA efflux inhibitors (1–4) to reduce the efflux activity in Mycobacterium smegmatis and Mycobacterium avium strains. All four compounds were able to inhibit efflux pumps […]
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Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Clonal expansion across the seas as seen through CPLP-TB database: A joint effort in cataloguing Mycobacterium tuberculosis genetic diversity in Portuguese-speaking countries

Genomic diversity of drug-resistant Mycobacterium tuberculosis isolates in Lisbon Portugal: Towards tuberculosis genomic epidemiology

Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa

Boosting Effect of 2-Phenylquinoline Efflux Inhibitors in Combination with Macrolides against Mycobacterium smegmatis and Mycobacterium avium

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