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Home / Archives for Inácio J

Draft genome sequence of a rare pigmented mycobacterium avium subsp. paratuberculosis type C strain

  • Authors: Amaro A, Barbosa P, Botelho A, Inácio J, Leao C, Pinto C, Ramos AM, Stevenson K, Usié A
  • Publication Year: 2017
  • Journal: Genome Announcements
  • Link: http://genomea.asm.org/content/5/41/e01066-17.full%20

Mycobacterium avium subsp. paratuberculosis is the causative agent of paratuberculosis. We report here the draft genome sequence of a rare pigmented M. avium subsp. paratuberculosis type C strain, comprising 58 contigs and having a genome size of 4,851,414 bp. The genome will assist in the execution of pigmentation and virulence studies on this mycobacterium.
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A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

  • Authors: Amaro A, Gomes J, Inácio J, Pereira da Fonseca I, Santos M, Santos-Gomes G
  • Publication Year: 2017
  • Journal: Molecular and Cellular Probes
  • Link: https://www.sciencedirect.com/science/article/pii/S0890850816301220?via%3Dihub

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% […]
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Novel Single Nucleotide Polymorphism-Based Assay for Genotyping Mycobacterium avium subsp Paratuberculosis

  • Authors: Bryant J, Goldstone RJ, Inácio J, Leao C, McLuckie J, Smith DG, Stevenson K
  • Publication Year: 2016
  • Journal: Journal Of Clinical Microbiology
  • Link: http://jcm.asm.org/content/54/3/556.abstract?utm_source=TrendMDJClinMicrobiol&utm_medium=TrendMDJClinMicrobiol&utm_campaign=TrendMD_JCMCLIN_1

Typing of Mycobacterium avium subspecies paratuberculosis strains presents a challenge, since they are genetically monomorphic and traditional molecular techniques have limited discriminatory power. The recent advances and availability of whole-genome sequencing have extended possibilities for the characterization of Mycobacterium avium subspecies paratuberculosis, and whole-genome sequencing can provide a phylogenetic context to facilitate global epidemiology studies. In this study, we developed a single […]
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Environmental distribution of Cryptococcus neoformans and C. Gattii around the Mediterranean basin

  • Authors: Akcaglar S, Al-Yasiri MH, Alonso B, Bertout S, Boekhout T, Caggiano G, Cerikcioglu N, Cogliati M, Colom MF, Criseo G, D'Amicis R, De Giglio O, Desnos-Ollivier M, Dipineto L, Dromer F, Ellabib MS, Ergin C, Ferreira-Paim K, Hagen F, Ilkit M, Inácio J, Kaya M, Korem M, Kwon-Chung KJ, Lockhart SR, Macci C, Mallié M, Marchese A, Martins Mda L, McCormick-Smith I, Meyer W, Mlinaric-Missoni E, Montagna MT, Nardoni S, Oliveri S, Pasquale V, Pereira C, Polacheck I, Ranque S, Rickerts V, Romeo O, Sampaio AC, Scopa A, Serio F, Susever S, Theelen B, Tintelnot K, Tore O, Tortorano AM, Trovato L, Varma A, Velegraki A, Vencà AC, Vezzulli L, Vieira ML, Zani A
  • Publication Year: 2016
  • Journal: FEMS Yeast Research
  • Link: https://academic.oup.com/femsyr/article/16/4/fow045/2680354

In order to elucidate the distribution of Cryptococcus neoformans and C. gattii in the Mediterranean basin, an extensive environmental survey was carried out during 2012-2015. A total of 302 sites located in 12 countries were sampled, 6436 samples from 3765 trees were collected and 5% of trees were found to be colonized by cryptococcal yeasts. […]
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Population diversity of Theileria annulata in Portugal

  • Authors: Amaro A, Gomes J, Inácio J, Pereira da Fonseca I, Pinto J, Salgueiro P, Santos-Gomes G, Shiels B, Tait A, Weir W
  • Publication Year: 2016
  • Journal: Infection Genetics and Evolution
  • Link: https://www.sciencedirect.com/science/article/pii/S1567134816301496?via%3Dihub

The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact […]
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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