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Home / Publicações / A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

  • Autores: Amaro A, Gomes J, Inácio J, Pereira da Fonseca I, Santos M, Santos-Gomes G
  • Ano de Publicação: 2017
  • Journal: Molecular and Cellular Probes
  • Link: https://www.sciencedirect.com/science/article/pii/S0890850816301220?via%3Dihub

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

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GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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