- Authors: Amaro A, Gomes J, Inácio J, Pereira da Fonseca I, Santos M, Santos-Gomes G
- Publication Year: 2017
- Journal: Molecular and Cellular Probes
- Link: https://www.sciencedirect.com/science/article/pii/S0890850816301220?via%3Dihub
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.
