Randomly amplified polymorphic DNA (RAPD) polymerase chain reaction assay for comparison of Schistosoma mansoni strains sensitive and resistant to praziquantel.
- Autores: Afonso A, Belo S, Calado M, Clemente I, Ferreira CC, Ferreira PM, Figueira AR, Grácio MA, Maurício I, Mendes T, Santos R
- Journal: Tropical Medicine & International Health
- Link: https://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=2&SID=2CbhBUDoSw8ZkFBEQLW&page=1&doc=1
Immunological and genetical analysis of combined therapy with artemisinin-praziquantel in Schistosoma mansoni in vivo.
Trichinella spiralis: Genome database searches for the presence and immunolocalization of protein disulphide isomerase family members
- Autores: Clemente I, Freitas CP, Mendes T, Novo C
- Ano de Publicação: 2014
- Journal: Journal of Helminthology
- Link: http://www.ncbi.nlm.nih.gov/pubmed/25475092
The formation of nurse cells in host muscle cells during Trichinella spiralis infection is a key step in the infective mechanism. Collagen trimerization is set up via disulphide bond formation, catalysed by protein disulphide isomerase (PDI). In T. spiralis, some PDI family members have been identified but no localization is described and no antibodies specific for T. spiralis PDIs are available. In this work, computational approaches were used to search for non-described PDIs in the T. spiralis genome database and to check the cross-reactivity of commercial anti-human antibodies with T. spiralis orthologues.
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