Mechanisms of Antibiotic Resistance in Salmonella: Efflux Pumps, Genetics, Quorum Sensing and Biofilm Formation.
- Authors: Amaral L, Fanning S, Martins M, McCusker M
- Journal: Letters in Drug Design & Discovery
- Link: https://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=4&SID=2ExAqmsJ7vIMOy1UmoG&page=1&doc=1
Multidrug resistance (MDR) to antibiotics presents a serious therapeutic problem in the treatment of bacterial infections. The importance of this mechanism of resistance in clinical settings is reflected in the increasing number of reports of multidrug resistant isolates. In Salmonella enterica, the most common etiological agent of food borne salmonellosis worldwide, MDR is becoming a major concern.
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Direct modification of bioactive phenothiazines by exposure to laser radiation.
- Authors: Amaral L, Andrei IR, Boni M, Fanning S, Martins A, Militaru A, Molnar J, Nastasa V, Pascu A, Pascu ML, Smarandache A, Staicu A, Viveiros M
- Journal: Recent Patents on Anti-Infective Drug Discovery
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Direct+modification+of+bioactive+phenothiazines+by+exposure+to+laser+radiation.
Whereas exposure of combinations of a phenothiazine and bacterium to incoherent UV increases the activity of the phenothiazine, exposure of the phenothiazine alone does not yield an increase of its activity. Because the laser beam energy is greater than that produced by the incoherent UV sources, exposure of phenothiazines to specific lasers may yield molecules with altered activities over that of the unexposed parent.
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An original deal for new molecule: reversal of efflux pump activity, a rational strategy to combat gram-negative resistant bacteria.
- Authors: Amaral L, Fanning S, Pagès JM
- Journal: Current Medicinal Chemistry
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=n+original+deal+for+new+molecule%3A+reversal+of+efflux+pump+activity%2C+a+rational+strategy+to+combat+gram-negative+resistant+bacteria
The worldwide dissemination of «multi-drug resistant» (MDR) pathogens has severely reduced the efficacy of our antibiotic arsenal and increased the frequency of therapeutic failure. MDR bacteria over-express efflux pumps and this active mechanism can extrude all classes of antibiotics from the cell.
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Ethidium bromide efflux by Salmonella: modulation by metabolic energy, pH, ions and phenothiazines
- Authors: Amaral L, Cerca P, Couto I, Fanning S, Machado L, Martins A, Pagès JM, Spengler G, Viveiros M
- Journal: International Journal of Antimicrobial Agents
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Ethidium+bromide+efflux+by+Salmonella%3A+modulation+by+metabolic+energy%2C+pH%2C+ions+and+phenothiazines
The main efflux pump of Salmonella enterica serotype Enteritidis, which obtains its energy for the extrusion of noxious agents from the proton-motive force, was studied with the aid of an ethidium bromide (EtBr) semi-automated method under conditions that define the role of metabolic energy, ions and pH in the extrusion of the universal substrate EtBr.
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