Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

HIGHLIGHTS • Disputed mutations were found in 21.9% of strains resistant to isoniazid alone, evidencing underestimation of multi-drug resistance. • 73.4% of multi-drug-resistant cases were linked to ongoing transmission events. • Intracluster acquisition of novel drug-resistance mutations was found, revealing important amplification of resistance. ABSTRACT Genomic-based surveillance on the occurrence of drug resistance and its […]
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In vitro and in silico analysis of the efficiency of tetrahydropyridines as drug efflux inhibitors in Escherichia coli

Authors: Almeida da Silva PE, Bonacorso HG, Cantos J, Carrion LL, Costa SS, Couto I, Fernandes LD, Junqueira E, Machado KS, Martins MA, Ramos DF, Seus VR, Silva L Jr, Viveiros M, von Groll A, Zanatta N
Publication Year: 2017
Journal: International Journal of Antimicrobial Agents
Link: http://www.ijaaonline.com/article/S0924-8579(17)30026-2/fulltext

The objectives of this study were to evaluate tetrahydropyridine derivatives as efflux inhibitors and to understand the mechanism of action of the compounds by in silico studies. Minimum inhibitory concentration (MIC) determination, fluorometric methods and docking simulations were performed. The compounds NUNL02, NUNL09 and NUNL10 inhibited efflux, and NUNL02 is very likely a substrate of […]
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Beyond extensively drug-resistant tuberculosis in Lisbon, Portugal: a case of linezolid resistance acquisition presenting as an iliopsoas abscess

Authors: Couto I, Machado D, Maltez F, Perdigão J, Pereira C, Portugal I, Silva C, Silva H, Viveiros M
Publication Year: 2016
Journal: International Journal of Antimicrobial Agents
Link: https://www.sciencedirect.com/science/article/pii/S0924857916302692?via%3Dihub

Authors: Amaral L, Hohmann J, Martins A, Molnar J, Vasas A, Viveiros M
Journal: International Journal of Antimicrobial Agents
Link: http://www.ncbi.nlm.nih.gov/pubmed/21411294

Several compounds isolated from the plant Carpobrotus edulis were evaluated for their activity against multidrug-resistant (MDR) bacteria and their efflux pump systems.
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Role of calcium in the efflux system of Escherichia coli.

Authors: Amaral L, Cerca P, Costa S, Machado L, Martins A, Spengler G, Viveiros M
Journal: International Journal of Antimicrobial Agents
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Role+of+calcium+in+the+efflux+system+of+Escherichia+coli.

Efflux of antibiotics by Escherichia coli AG100 is performed by a variety of efflux pumps, ensuring survival of the bacterium in widely diverse media. At pH 5, efflux is independent of metabolic energy during the period of time the assay is conducted; at pH 8 it is totally dependent upon metabolic energy.
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Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

In vitro and in silico analysis of the efficiency of tetrahydropyridines as drug efflux inhibitors in Escherichia coli

Beyond extensively drug-resistant tuberculosis in Lisbon, Portugal: a case of linezolid resistance acquisition presenting as an iliopsoas abscess

Antibacterial properties of compounds isolated from Carpobrotus edulis.

Role of calcium in the efflux system of Escherichia coli.

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