GHTM

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Home / Archives for Pacheco T

Contribution of efflux to colistin heteroresistance in a multidrug resistant acinetobacter baumannii clinical isolate

  • Authors: Machado D, Antunes J, Simoes A, Perdigão J, Couto I, McCusker M, Martins M, Portugal I, Pacheco T, Batista J, Toscano C, Viveiros M
  • Publication Year: 2018
  • Journal: Journal of Medical Microbiology
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/29717972

PURPOSE: The mechanisms underlying colistin heteroresistance in Acinetobacter baumannii are not fully understood. Here, we investigated the role of efflux in colistin-heteroresistant populations of a multidrug-resistant (MDR) A. baumannii clinical isolate. METHODOLOGY: Three colistin-resistant A. baumannii strain variants isolated from the same clinical sample were studied for the presence of heteroresistance to colistin by drug […]
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Emergence of high-level colistin resistance in an acinetobacter baumannii clinical isolate mediated by inactivation of the global regulator H-NS

  • Authors: Deveson Lucas D, Crane B, Wright A, Han ML, Moffatt J, Bulach D, Gladman SL, Powell D, Aranda J, Seemann T, Machado D, Pacheco T, Marques T, Viveiros M, Nation R, Li J, Harper M, Boyce JD
  • Publication Year: 2018
  • Journal: Antimicrobial Agents and Chemotherapy
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/29712662

Colistin is a crucial last-line drug used for the treatment of life-threatening infections caused by multidrug-resistant strains of the Gram-negative bacterium Acinetobacter baumannii However, colistin-resistant A. baumannii isolates can still be isolated following failed colistin therapy. Resistance is most often mediated by the addition of phosphoethanolamine (pEtN) to lipid A by PmrC, following missense mutations in the pmrCAB operon encoding PmrC […]
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Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method.

  • Authors: Amaral L, Costa SS, Couto I, Fanning S, Martins M, Pacheco T, Pagès JM, Viveiros M
  • Journal: In vivo
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Identification+of+efflux+pump+mediated+multi-drug+resistant+bacteria+by+the+ethidium+bromide-agar+cartwheel+method

BACKGROUND/AIM:
Bacterial multidrug resistance may be mediated by the overexpression of efflux pumps. Conventional evaluation of efflux activity using efflux pump substrates, such as ethidium bromide, requires specialised instrumentation. The agar-based method, previously reported, has been modified to evaluate as many as twelve bacterial strains and has been termed the ethidium bromide-agar cartwheel method.
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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