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Home / Archives for Lopes A

Prevalence of gonorrhea and chlamydia in a community clinic for men who have sex with men in Lisbon, Portugal

  • Autores: Ribeiro S, de Sousa D, Medina D, Castro R, Lopes A, Rocha M
  • Journal: International journal of STD & AIDS
  • Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732818/

Men who have sex with men (MSM) are at greater risk for sexually transmitted infections (STIs). Data on MSM chlamydia and gonorrhea prevalence estimates and associated risk factors are scarce. To our knowledge, this is the first study to describe the prevalence and the determinants of both chlamydia and gonorrhea infections in MSM in Portugal. […]
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Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men

  • Autores: Calado J, Castro R, Lopes A, Campos MJ, Rocha M, Pereira F
  • Ano de Publicação: 2019
  • Journal: International Journal of Infectious Diseases
  • Link: https://www.researchgate.net/publication/328766979_Antimicrobial_resistance_and_molecular_characteristics_of_Neisseria_gonorrhoeae_isolates_from_MSM

Objectives: To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm) and spectinomycin (Spt). To verify the presence of Mutations in resistant genes. Methods: Antibiotic susceptibility testing was carried out by Etest method in 30 N. gonorrhoeae isolates collected from MSM population. PCR and […]
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Characterization of an insect-specific flavivirus (OCFVPT) co-isolated from Ochlerotatus caspius collected in southern Portugal along with a putative new Negev-like virus

  • Autores: Abecasis A, de Almeida AP, de Matos AP, Esteves A, Lopes A, Parreira R, Piedade J
  • Ano de Publicação: 2013
  • Journal: Virus Genes
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/23877720

We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. The RNA genome of this virus, tentatively designated OCFVPT, for O. caspius flavivirus from Portugal, encodes a polyprotein showing all the features expected for a flavivirus. As frequently observed for ISF, the viral genomes seems to encode a putative Fairly Interesting Flavivirus ORF (FIFO)-like product, the synthesis of which would occur as a result of a -1 translation frameshift event. OCFVPT was isolated in the C6/36 Stegomyia albopicta (= Aedes albopictus) cell line where it replicates rapidly, but failed to replicate in Vero cells in common with other ISFs.
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A divergent insect-specific flavivirus that causes overt cytopathic effect in a mosquito cell-line

  • Autores: Almeida AP, Alves de Matos AP, Duque Ferreira D, Esteves A, Lopes A, Parreira R, Piedade J
  • Ano de Publicação: 2013
  • Journal: Microscopy and Microanalysis
  • Link: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8964978&fileId=S1431927613001049

This work reports the initial characterization, including near full-length sequence analysis, of an ISF which seems to form a divergent lineage within the ISF radiation, as indicated by phylogenetic analysis based on ac multiple sequence alignment of the NS5 flavivirus coding sequence.
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Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice

  • Autores: Borrego M, Codices V, de Sousa B, Lopes A, Martins C, Matos O, Novo C, Pinho M
  • Ano de Publicação: 2013
  • Journal: Acta Parasitologica
  • Link: http://link.springer.com/article/10.2478%2Fs11686-013-0113-2

Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22.
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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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