- Autores: Calado J, Castro R, Lopes A, Campos MJ, Rocha M, Pereira F
- Ano de Publicação: 2019
- Journal: International Journal of Infectious Diseases
- Link: https://www.researchgate.net/publication/328766979_Antimicrobial_resistance_and_molecular_characteristics_of_Neisseria_gonorrhoeae_isolates_from_MSM
Objectives: To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm) and spectinomycin (Spt). To verify the presence of Mutations in resistant genes. Methods: Antibiotic susceptibility testing was carried out by Etest method in 30 N. gonorrhoeae isolates collected from MSM population. PCR and DNA sequencing were performed to identify mutations within penA, mtrR, gyrA and parC genes in intermediate or full resistance isolates. Results: N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73%), Cfm (3%), Tet (60%), Cip (37%) and Azm (13%). One isolate CfmR presented a PBP2 mosaic XXXIV. All PenI and PenR isolates (except at PBP2 mosaic) presented a D345a in PBP2 and all CipR isolates had a S91F at gyrA gene together with mutations at parC gene. All intermediate or full resistance isolates to substrates of MtrCDE efflux pump had an A39T or G45D mutation in mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a N. meningitidis-like mtrR promoter sequence. Conclusions: The results of this study are consisting with the findings of other authors and reinforce the importance of developing new therapeutic options in a short time period.