A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

Authors: Calzetta M, Perugini E, Seixas G, Sousa CA, Guelbeogo WM, Sagnon N, Della Torre A, Pinto J, Pombi M, Mancini E
Publication Year: 2018
Journal: Medical and Veterinary Entomology
Link: https://www.ncbi.nlm.nih.gov/pubmed/29344968

Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium […]
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First evidence of resistance to pyrethroid insecticides in Italian Aedes albopictus populations 26 years after invasion

Authors: Pichler V, Bellini R, Veronesi R, Arnoldi D, Rizzoli A, Lia RP, Otranto D, Montarsi F, Carlin S, Ballardini M, Antognini E, Salvenini M, Brianti E, Gaglio G, Manica M, Cobre P, Serini P, Velo E, Vontas J, Kioulos I, Pinto J, Della Torre A, Caputo B
Publication Year: 2018
Journal: Pest Management Science
Link: https://onlinelibrary.wiley.com/doi/epdf/10.1002/ps.4840%20

BACKGROUND: Aedes albopictus has spread during the last few decades all over the world. This has increased significantly the risk of exotic arbovirus transmission (e.g. chikungunya, dengue, and Zika) also in temperate areas, as demonstrated by the Chikungunya 2007 and 2017 outbreaks in northeastern and central Italy. Insecticides are an important tool for limiting the […]
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Remarkable diversity of intron-1 of the para voltage-gated sodium channel gene in an Anopheles gambiae/Anopheles coluzzii hybrid zone

Authors: Caputo B, Conway DJ, Della Torre A, Fanello C, Mancini E, Nwakanma DC, Petrarca V, Pinto J, Santolamazza F, Weetman D
Publication Year: 2015
Journal: Malaria Journal
Link: http://www.ncbi.nlm.nih.gov/pubmed/25604888

BACKGROUND: Genomic differentiation between Anopheles gambiae and Anopheles coluzzii–the major malaria vectors in sub-Saharan Africa–is localized into large “islands” toward the centromeres of chromosome-X and the two autosomes. Linkage disequilibrium between these genomic islands was first detected between species-specific polymorphisms within ribosomal DNA genes (IGS-rDNA) on the X-chromosome and a single variant at position 702 […]
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The last bastion? X chromosome genotyping of Anopheles gambiae species pair males from a hybrid zone reveals complex recombination within the major candidate ‘genomic island of speciation’

Authors: Caputo B, Della Torre A, Dinis J, Mancini E, Petrarca V, Pichler V, Pinto J, Pombi M, Rodrigues A, Steen K, Vicente JL, Weetman D
Publication Year: 2016
Journal: Molecular Ecology
Link: http://onlinelibrary.wiley.com/doi/10.1111/mec.13840/pdf

Speciation with gene flow may be aided by reduced recombination helping to build linkage between genes involved in the early stages of reproductive isolation. Reduced recombination on chromosome X has been implicated in speciation within the Anopheles gambiae complex, species of which represent the major Afrotropical malaria vectors. The most recently diverged, morphologically indistinguishable, species […]
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Genetic isolation within the malaria mosquito Anopheles melas.

Authors: Athrey G, Caccone A, Costantini C, Deitz KC, Della Torre A, Jawara M, Kengne P, Kiszewski AE, Matias A, Overgaard HJ, Petrarca V, Pinto J, Reddy MR, Slotman MA
Journal: Molecular Ecology
Link: http://www.ncbi.nlm.nih.gov/pubmed/22882458

Anopheles melas is a brackish water-breeding member of the Anopheles gambiae complex that is distributed along the coast of West Africa and is a major malaria vector within its range. Because little is known about the population structure of this species, we analysed 15 microsatellite markers and 1161 bp of mtDNA in 11 A.
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A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

First evidence of resistance to pyrethroid insecticides in Italian Aedes albopictus populations 26 years after invasion

Remarkable diversity of intron-1 of the para voltage-gated sodium channel gene in an Anopheles gambiae/Anopheles coluzzii hybrid zone

The last bastion? X chromosome genotyping of Anopheles gambiae species pair males from a hybrid zone reveals complex recombination within the major candidate ‘genomic island of speciation’

Genetic isolation within the malaria mosquito Anopheles melas.

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