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Home / Archives for Medical and Veterinary Entomology

Molecular detection of zoonotic filarioids in Culex spp. from Portugal

  • Authors: Ranju Ravindran Santhakumari Manoj, Maria Stefania Latrofa, Maria Alfonsa Cavalera, Jairo Alfonso Mendoza-Roldan, Carla Maia, Domenico Otranto
  • Publication Year: 2021
  • Journal: Medical and Veterinary Entomology, 35(3), pp 468 - 477
  • Link: https://doi.org/10.1111/mve.12524

ABSTRACT To investigate the role of dipterans in the transmission of Onchocerca lupi and other zoonotic filarioids, samples were collected from different sites in Algarve, southern Portugal, morphologically identified and molecularly tested for filarioids. Culex sp. (72.8%) represented the predominant genus followed by Culicoides sp. (11.8%), Ochlerotatus sp. (9.7%), Culiseta sp. (4.5%), Aedes sp. (0.9%) and Anopheles sp. (0.3%). Nineteen (2.8%) specimens scored positive for filarioids, with Culex pipiens quinquefasciatus (2%) […]
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A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

  • Authors: Calzetta M, Perugini E, Seixas G, Sousa CA, Guelbeogo WM, Sagnon N, Della Torre A, Pinto J, Pombi M, Mancini E
  • Publication Year: 2018
  • Journal: Medical and Veterinary Entomology
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/29344968

Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium […]
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The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis

  • Authors: Campino L, Courtenay O, Cristóvão JM, Fiorentino E, Gradoni L, Gramiccia M, Kostalova T, Lestinova T, Maia C, Oliva G, Polanska N, Scalone A, Sumova P, Veronesi F, Vlkova M, Volf P, Willen L
  • Publication Year: 2017
  • Journal: Medical and Veterinary Entomology
  • Link: https://onlinelibrary.wiley.com/doi/abs/10.1111/mve.12192%20

The frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) […]
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Molecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileri

  • Authors: Almeida AP, Belo S, Calado MM, de Pinho Mixao V, Mauricio IL, Mendes AM, Novo MT
  • Publication Year: 2016
  • Journal: Medical and Veterinary Entomology
  • Link: https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12179

Wolbachia pipientis (Rickettsiales: Rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. However, the effect of its co-infection on vector competence for Dirofilaria immitis (Spirurida: Onchocercidae) in the wild has not been investigated. This study aimed to screen vectors of D. immitis for wPip, to characterize these, and to investigate a possible association between the […]
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Analysis of the sporozoite ELISA for estimating infection rates in Mozambican anophelines

  • Authors: Charlwood JD, Cuamba N, Pinto J, Tomas EVE
  • Publication Year: 2015
  • Journal: Medical and Veterinary Entomology
  • Link: https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12084%20

Comparisons were undertaken to investigate cost-effective methods of implementing the enzyme-linked immunosorbent assay (ELISA) for sporozoite determination in anophelines when large numbers require processing. Comparisons between ELISA plate reader and visual assessments were performed with Anopheles funestus and Anopheles gambiae s.l. (Diptera: Culicidae), as were comparisons between whole-body mosquito samples, heads and thoraces, and abdomens […]
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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