A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes
- Authors: Calzetta M, Perugini E, Seixas G, Sousa CA, Guelbeogo WM, Sagnon N, Della Torre A, Pinto J, Pombi M, Mancini E
- Publication Year: 2018
- Journal: Medical and Veterinary Entomology
- Link: https://www.ncbi.nlm.nih.gov/pubmed/29344968
Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium […]
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