Nested and Multiplex Real-Time PCR Using Dual-Labeled Probes: Detecting and Discriminating Mycobacterium tuberculosis Complex Members in Cultures and Animal Tissues
- Authors: Costa P, Couto I, Inácio J, Viveiros M
- Publication Year: 2015
- Journal: Methods in Molecular Biology
- Link: http://www.ncbi.nlm.nih.gov/pubmed/25399092
Members of the Mycobacterium tuberculosis complex (MTC) are causative agents of tuberculosis (TB) in both humans and animals. In the last two decades, the accumulating knowledge of the nucleotide sequences of several genes, and of the whole genomes, of MTC members has allowed the development of novel molecular assays able to detect and discriminate between these species.
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