GHTM

Global Health and Tropical Medicine

  • GHTM
    • Vision
    • Mission
    • Governance
    • Scientific Advisory Board
  • News
    • Outreach
    • Events
      • GHTM Sessions
      • Workshops
    • Articles
    • Jobs
  • Research
    • Cross-cutting issues
      • Global Pathogen Dispersion and Population Mobility
      • Drug Discovery and Drug Resistance
      • Diagnostics
      • Public Health Information
      • Fair Research Partnerships
    • Research Groups
      • PPS – Population health, policies and services
      • THOP – TB, HIV and opportunistic diseases and pathogens
      • VBD – Vector borne diseases and pathogens
      • IHC – Individual health care
    • Research in numbers
      • 2020
      • 2019
      • 2018
      • 2017
    • Projects
      • Ongoing Projects
    • Members
      • Population health, policies and services
        • PPS PhD members
        • PPS non PhD members
      • TB, HIV and opportunistic diseases and pathogens
        • THOP PhD members
        • THOP non PhD members
      • Vector-borne diseases and pathogens
        • VBD PhD members
        • VBD non PhD members
      • Individual Health Care
        • IHC PhD members
        • IHC non PhD members
      • Technical / administrative support
  • Publications
  • Education
    • Master Theses
    • PhD Theses
  • Services
Home / Archives for Methods in Molecular Biology

Measuring Efflux and Permeability in Mycobacteria – chapter of «Mycobacteria Protocols»

  • Autores: Liliana Rodrigues, José A Aínsa, Miguel Viveiros
  • Ano de Publicação: 2021
  • Journal: Mycobacteria Protocols, 231-245, part of the book series, Methods in Molecular Biology, 2314
  • Link: https://doi.org/10.1007/978-1-0716-1460-0_9

ABSTRACT Mycobacteria are intrinsically resistant to most antimicrobials, which is generally attributed to the impermeability of their cell wall that considerably limits drug uptake. Moreover, like in other pathogenic bacteria, active efflux systems have been widely characterized from diverse mycobacterial species in laboratory conditions, showing that they can promote resistance by extruding noxious compounds prior […]
Ler mais

Phylogenetic Studies

  • Autores: Kuhls K, Maurício I
  • Ano de Publicação: 2019
  • Journal: Methods in Molecular Biology
  • Link: https://europepmc.org/abstract/med/30980297

Phylogenetics is an important component of the systems biology approach. Knowledge about evolution of the genus Leishmania is essential to understand various aspects of basic biology of these parasites, such as parasite-host or parasite-vector relationships, biogeography, or epidemiology. Here, we present a comprehensive guideline for performing phylogenetic studies based on DNA sequence data, but with […]
Ler mais

Functional genomics of tick vectors challenged with the cattle parasite Babesia bigemina

  • Autores: Antunes S, de la Fuente J, Domingos A, Villar M
  • Ano de Publicação: 2015
  • Journal: Methods in Molecular Biology
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/25399115

Ticks are obligate hematophagous ectoparasites considered as vectors of animal diseases, having a huge economic impact in cattle industry. Babesia spp. are tick-borne pathogens that cause a disease called babesiosis in a wide range of animals and in humans. Control of tick infestations is mainly based on the use of acaricides, which have limited efficacy […]
Ler mais

Use of zymography in trypanosomiasis studies

  • Autores: Monte JF, Monteiro J, Moreno CJ, Rimoldi AR, Rocha HA, Silva MS
  • Ano de Publicação: 2017
  • Journal: Methods in Molecular Biology
  • Link: https://link.springer.com/protocol/10.1007%2F978-1-4939-7111-4_20

Zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. Samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (SDS) and a specific substrate (gelatin, casein, collagen, etc.). Then proteins are allowed to […]
Ler mais

Measuring efflux and permeability in mycobacteria

  • Autores: Ainsa JA, Rodrigues L, Viveiros M
  • Ano de Publicação: 2015
  • Journal: Mycobacteria Protocols, pp 227-239, part of the book series, Methods in Molecular Biology, 1285
  • Link: https://link.springer.com/protocol/10.1007/978-1-4939-2450-9_13

http://www.ncbi.nlm.nih.gov/pubmed/25779319
Ler mais

  • 1
  • 2
  • Next Page »

About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

Contacts

Rua da Junqueira, 100
1349-008 Lisboa
Portugal
+351 213 652 600
+351 213 632 105

  • Facebook
  • YouTube

Subscribe Newsletter

  • How to get to GHTM/IHMT
  • GHTM Sessions
  • Research Groups
  • Cross-cutting issues
© Copyright 2023 IHMT-UNL Todos os Direitos Reservados.
  • Universidade Nova de Lisboa
  • Fundação para a Ciência e a Tecnologia

    Project UID/Multi/04413/2013