Use of zymography in trypanosomiasis studies
- Authors: Monte JF, Monteiro J, Moreno CJ, Rimoldi AR, Rocha HA, Silva MS
- Publication Year: 2017
- Journal: Methods in Molecular Biology
- Link: https://link.springer.com/protocol/10.1007%2F978-1-4939-7111-4_20
Zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. Samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (SDS) and a specific substrate (gelatin, casein, collagen, etc.). Then proteins are allowed to […]
Read More