Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance.

Authors: Amaral L, Couto I, Ramos J, Rodrigues L, Viveiros M
Journal: BMC Microbiology
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Ethidium+bromide+transport+across+Mycobacterium+smegmatis+cell-wall%3A+correlation+with+antibiotic+resistance

BACKGROUND:
Active efflux systems and reduced cell-wall permeability are considered to be the main causes of mycobacterial intrinsic resistance to many antimicrobials. In this study, we have compared the Mycobacterium smegmatis wild-type strain mc2155 with knockout mutants for porins MspA (the main porin of M. smegmatis) and MspC, the efflux pump LfrA (the main efflux pump system of M. smegmatis) and its repressor LfrR for their ability to transport ethidium bromide (EtBr) on a real-time basis.
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Ethidium bromide efflux by Salmonella: modulation by metabolic energy, pH, ions and phenothiazines

Authors: Amaral L, Cerca P, Couto I, Fanning S, Machado L, Martins A, Pagès JM, Spengler G, Viveiros M
Journal: International Journal of Antimicrobial Agents
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Ethidium+bromide+efflux+by+Salmonella%3A+modulation+by+metabolic+energy%2C+pH%2C+ions+and+phenothiazines

The main efflux pump of Salmonella enterica serotype Enteritidis, which obtains its energy for the extrusion of noxious agents from the proton-motive force, was studied with the aid of an ethidium bromide (EtBr) semi-automated method under conditions that define the role of metabolic energy, ions and pH in the extrusion of the universal substrate EtBr.
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Identification of nontuberculous mycobacteria in clinical samples using molecular methods: a 3-year study

Nontuberculous mycobacteria (NTM) are being increasingly isolated in clinical laboratories and present technical and therapeutic challenges.
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Identification of the plasmid-encoded qacA efflux pump gene in meticillin-resistant Staphylococcus aureus (MRSA) strain HPV107, a representative of the MRSA Iberian clone

Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial bacterium for which prevention and control measures consist mainly of the application of biocides with antiseptic and disinfectant activity.
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Rational Design and Synthesis of Thioridazine Analogues as Enhancers of the Antituberculosis Therapy

Authors: Azzali E, Costa SS, Costantino G, Couto I, Machado D, Pieroni M, Viveiros M
Publication Year: 2015
Journal: Journal of Medicinal Chemistry
Link: https://pubs.acs.org/doi/10.1021/acs.jmedchem.5b00428

Tuberculosis, caused by Mycobacterium tuberculosis, is still one of the leading infectious diseases globally. Therefore, novel approaches are needed to face this disease. Efflux pumps are known to contribute to the emergence of M. tuberculosis drug resistance. Thioridazine has shown good anti-TB properties both in vitro and in vivo, likely due to its capacity to […]
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Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance.

Ethidium bromide efflux by Salmonella: modulation by metabolic energy, pH, ions and phenothiazines

Identification of nontuberculous mycobacteria in clinical samples using molecular methods: a 3-year study

Identification of the plasmid-encoded qacA efflux pump gene in meticillin-resistant Staphylococcus aureus (MRSA) strain HPV107, a representative of the MRSA Iberian clone

Rational Design and Synthesis of Thioridazine Analogues as Enhancers of the Antituberculosis Therapy

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