Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease

Authors: Esteves LM, Bulhões SM, Branco CC, Carreira T, Vieira ML, Gomes-Solecki M, Mota-Vieira L
Publication Year: 2018
Journal: Scientific Reports
Link: https://www.ncbi.nlm.nih.gov/pubmed/29907838

Currently, direct detection of Leptospira can be done in clinical laboratories by conventional and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and urine from the same patient (202 patients) presenting at the hospital in an area endemic for leptospirosis using qRT-PCR followed by high resolution melting (HRM) analysis. The […]
Read More

Human Leptospirosis: Seroreactivity and Genetic Susceptibility in the Population of Sao Miguel Island (Azores, Portugal)

We conducted, in 2011, a retrospective hospital-based case-control study in the São Miguel Island population (Azores archipelago). In order to determine the seropositivity against pathogenic Leptospira after the first episode of leptospirosis, we performed a serological evaluation in 97 unrelated participants diagnosed with leptospirosis between 1992 and 2011.
Read More

Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease

Human Leptospirosis: Seroreactivity and Genetic Susceptibility in the Population of Sao Miguel Island (Azores, Portugal)

About GHTM

Contacts

Map