Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease

• Authors: Esteves LM, Bulhões SM, Branco CC, Carreira T, Vieira ML, Gomes-Solecki M, Mota-Vieira L
• Publication Year: 2018
• Journal: Scientific Reports
• Link: https://www.ncbi.nlm.nih.gov/pubmed/29907838

Currently, direct detection of Leptospira can be done in clinical laboratories by conventional and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and urine from the same patient (202 patients) presenting at the hospital in an area endemic for leptospirosis using qRT-PCR followed by high resolution melting (HRM) analysis. The […]
Read More