GHTM

Global Health and Tropical Medicine

Home / Archives for Ricardo Parreira

Virological screening of wild and farmed fish species: Application of innovative Taqman multiplex real-time PCR protocols

ABSTRACT Global demand for seafood is growing and fish stock maintenance becomes a pressing issue, making aquaculture production of capital importance in terms of fish resources sustainability. Therefore, fish disease outbreaks are a significant constraint to fisheries trade and aquaculture production, affecting economic development worldwide. In this study, a virological screening in wild and farmed fish […]
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Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

REGIONAL REPORT ABSTRACT The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence […]
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Leishmania infantum strains from cats are similar in biological properties to canine and human strains

HIGHLIGHTS • Feline Leishmania strains are phenotypically similar to those from humans and dogs. • Feline, canine, and human strains have a identical susceptibility to drugs. • Parasites isolated from cats can infect feline, canine, and human macrophages. • Feline macrophages are permissive to strains isolated from dogs and humans. ABSTRACT Zoonotic visceral leishmaniosis is a […]
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Giardia duodenalis infection in dogs from the metropolitan area of Lisbon, Portugal: prevalence, genotyping and associated risk factors

ABSTRACT Giardia duodenalis is a cosmopolitan enteric protozoan that affects a wide range of vertebrates, including humans and dogs. Genetic characterisation reveals eight different assemblages, with A and B having been found mainly in humans and several other animals, and thus considered potentially zoonotic, while C and D are adapted to infect dogs. This study […]
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Genetic lineage characterization and spatiotemporal dynamics of classical insect-specific flaviviruses: outcomes and limitations

HIGHLIGHTS • Complete and partial cISF sequences were characterized using multiple analyses. • cISF sequences show low diversity and entropy, and evolve under purifying selection. • Recombination does not seem to impact the evolution of cISF extensively. • Different datasets displayed high phylogenetic, but mostly low temporal signals. • Spatiotemporal dispersal analyses suggested cISF distribution […]
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GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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