GHTM

Global Health and Tropical Medicine

  • GHTM
    • Vision
    • Mission
    • Governance
    • Scientific Advisory Board
  • News
    • Outreach
    • Events
      • GHTM Sessions
      • Workshops
    • Articles
    • Jobs
  • Research
    • Cross-cutting issues
      • Global Pathogen Dispersion and Population Mobility
      • Drug Discovery and Drug Resistance
      • Diagnostics
      • Public Health Information
      • Fair Research Partnerships
    • Research Groups
      • PPS – Population health, policies and services
      • THOP – TB, HIV and opportunistic diseases and pathogens
      • VBD – Vector borne diseases and pathogens
      • IHC – Individual health care
    • Research in numbers
      • 2020
      • 2019
      • 2018
      • 2017
    • Projects
      • Ongoing Projects
    • Members
      • Population health, policies and services
        • PPS PhD members
        • PPS non PhD members
      • TB, HIV and opportunistic diseases and pathogens
        • THOP PhD members
        • THOP non PhD members
      • Vector-borne diseases and pathogens
        • VBD PhD members
        • VBD non PhD members
      • Individual Health Care
        • IHC PhD members
        • IHC non PhD members
      • Technical / administrative support
  • Publications
  • Education
    • Master Theses
    • PhD Theses
  • Services
Home / Publicações / Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

  • Autores: Diogo Filipe, Ricardo Parreira, André Pereira, Nuno Galvão, José Manuel Cristóvão, Mónica Nunes, Maria Luísa Vieira, Lenea Campino, Carla Maia
  • Ano de Publicação: 2021
  • Journal: Veterinary Parasitology: Regional Studies and Reports, vol 24, art 100551
  • Link: https://doi.org/10.1016/j.vprsr.2021.100551

REGIONAL REPORT

ABSTRACT

The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.

HIGHLIGHTS

• 16S-rDNA seems more suitable to identify hard ticks to the species level using BLASTn.
• 16S-rDNA and COX1 molecular markers suitable to identify ticks to a species level.
• Phylogenetic analyses confirmed the results suggested by BOLD-ID and/or BLASTn results.

KEYWORDS

Hard ticks; DNA barcoding; Phylogeny; Sequence analysis.

Share this:

  • Click to share on Facebook (Opens in new window)
  • Click to share on Twitter (Opens in new window)
  • Click to share on LinkedIn (Opens in new window)
  • Click to share on Pinterest (Opens in new window)
  • Click to share on WhatsApp (Opens in new window)
  • Click to print (Opens in new window)

Events

“Python Applied to Biomedical Sciences course allowed students to understand the applications of this programming language”

  The first edition of the course "Python Applied to Biomedical Sciences" was organized and … [Read More...]

Status of COVID-19 Transmission in Secondary Schools in Europe – First results and evidence gathered within the framework of the European EUCARE project with the participation of GHTM

EUCARE Project – Status of COVID-19 Transmission in Secondary Schools in Europe – First results and … [Read More...]

Members of PPS-GHTM Research Group attended the 17th World Congress on Public Health

  Researchers, Master and PhD students from the PPS-GHTM Research Group attended the 17th … [Read More...]

Erasmus+: IHMT-NOVA welcomes two researchers from the University of Cape Verde

IHMT-NOVA welcomes two researchers from the University of Cape Verde. Between 5th and 9th of June, … [Read More...]

Call for PhD Studentships

The Institute of Hygiene and Tropical Medicine (IHMT), Universidade Nova de Lisboa (NOVA), through … [Read More...]

IHMT | GHTM – APPLICATIONS ARE OPEN!

IHMT | GHTM - Applications are open for three research vacancies:   One position - PhD … [Read More...]

About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

Contacts

Rua da Junqueira, 100
1349-008 Lisboa
Portugal
+351 213 652 600
+351 213 632 105

  • Facebook
  • YouTube

Subscribe Newsletter

  • How to get to GHTM/IHMT
  • GHTM Sessions
  • Research Groups
  • Cross-cutting issues
© Copyright 2023 IHMT-UNL Todos os Direitos Reservados.
  • Universidade Nova de Lisboa
  • Fundação para a Ciência e a Tecnologia

    Project UID/Multi/04413/2013