Virological screening of wild and farmed fish species: Application of innovative Taqman multiplex real-time PCR protocols

Authors: Andreia Filipa-Silva, Ricardo Parreira, Mónica Nunes, Maria Teresa Barreto Crespo
Publication Year: 2021
Journal: Aquaculture, vol 54015, art 736693
Link: https://doi.org/10.1016/j.aquaculture.2021.736693

ABSTRACT Global demand for seafood is growing and fish stock maintenance becomes a pressing issue, making aquaculture production of capital importance in terms of fish resources sustainability. Therefore, fish disease outbreaks are a significant constraint to fisheries trade and aquaculture production, affecting economic development worldwide. In this study, a virological screening in wild and farmed fish […]
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Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Authors: Diogo Filipe, Ricardo Parreira, André Pereira, Nuno Galvão, José Manuel Cristóvão, Mónica Nunes, Maria Luísa Vieira, Lenea Campino, Carla Maia
Publication Year: 2021
Journal: Veterinary Parasitology: Regional Studies and Reports, vol 24, art 100551
Link: https://doi.org/10.1016/j.vprsr.2021.100551

REGIONAL REPORT ABSTRACT The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence […]
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Development of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples

Authors: Andreia Filipa-Silva, Mónica Nunes, Ricardo Parreira, Maria Teresa Barreto Crespo
Publication Year: 2021
Journal: Microorganisms, 9(6), art 1149
Link: https://doi.org/10.3390/microorganisms9061149

ABSTRACT Human enteric viruses such as norovirus (NoV) and hepatitis A virus (HAV) are some of the most important causes of foodborne infections worldwide. Usually, infection via fish consumption is not a concern regarding these viruses, since fish are mainly consumed cooked. However, in the last years, raw fish consumption has become increasingly common, especially […]
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Virological screening of wild and farmed fish species: Application of innovative Taqman multiplex real-time PCR protocols

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Development of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples

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