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Home / Publicações / Virological screening of wild and farmed fish species: Application of innovative Taqman multiplex real-time PCR protocols

Virological screening of wild and farmed fish species: Application of innovative Taqman multiplex real-time PCR protocols

  • Autores: Andreia Filipa-Silva, Ricardo Parreira, Mónica Nunes, Maria Teresa Barreto Crespo
  • Ano de Publicação: 2021
  • Journal: Aquaculture, vol 54015, art 736693
  • Link: https://doi.org/10.1016/j.aquaculture.2021.736693

ABSTRACT

Global demand for seafood is growing and fish stock maintenance becomes a pressing issue, making aquaculture production of capital importance in terms of fish resources sustainability. Therefore, fish disease outbreaks are a significant constraint to fisheries trade and aquaculture production, affecting economic development worldwide. In this study, a virological screening in wild and farmed fish populations was performed by two TaqMan multiplex real-time PCR assays developed to identify/quantify, in a single run, four viruses responsible for major economic losses in fisheries, namely: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), viral haemorrhagic septicaemia virus (VHSV) and viral nervous necrosis virus (VNNV). A total of 323 fish were assessed, being the majority from four species from the Atlantic coast: gilthead seabream and seabass, two extensively farmed species in aquaculture and captured in the wild, and sardine and Atlantic horse mackerel, two of the most consumed fish species in Portugal. The genomes of IPNV (genogroup II) and VHSV (genogroup Ie) were detected in one pool of gilthead seabream and one pool of Atlantic horse mackerel, confirming the importance of molecular tools for viral screening, especially in asymptomatic hosts, since viral infections may impart major economic losses to the fish industry.

 

HIGHLIGHTS

• Two multiplex qPCR assays were developed to detect/quantify four fish viruses.
• Both multiplex assays showed the same sensitivity and reproducibility.
• IPNV/IHNV and VHSV/VNNV detected in a single run by two multiplex qPCR protocols.
• IPNV and VHSV genomes detected in asymptomatic wild fish from the Portuguese coast.

KEYWORDS

Pathogenic fish viruses; Multiplex TaqMan qPCR assays; Infectious pancreatic necrosis virus (IPNV); Infectious hematopoietic necrosis virus (IHNV); Viral Haemorrhagic Septicaemia virus (VHSV); Viral nervous necrosis virus (VNNV).

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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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