Genetic lineage characterization and spatiotemporal dynamics of classical insect-specific flaviviruses: outcomes and limitations

Authors: Paulo Morais, Nídia S Trovão, Ana Abecasis, Ricardo Parreira
Publication Year: 2021
Journal: Virus Research, 303, art 198507
Link: https://doi.org/10.1016/j.virusres.2021.198507

HIGHLIGHTS • Complete and partial cISF sequences were characterized using multiple analyses. • cISF sequences show low diversity and entropy, and evolve under purifying selection. • Recombination does not seem to impact the evolution of cISF extensively. • Different datasets displayed high phylogenetic, but mostly low temporal signals. • Spatiotemporal dispersal analyses suggested cISF distribution […]
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A diverse assemblage of RNA and DNA viruses found in mosquitoes collected in southern Portugal

Authors: Silva M, Morais P, Maia C, de Sousa CB, de Almeida APG, Parreira R
Publication Year: 2019
Journal: Virus Research
Link: https://www.ncbi.nlm.nih.gov/pubmed/31560965

This work describes the detection and partial characterization of mosquito-borne virus genomic sequences, based on the analysis of mosquitoes collected from the Spring to Fall of 2018 in the Algarve (southern Portugal). The viral survey that was carried out using multiple primer sets disclosed the presence of both RNA and DNA viral sequences in these […]
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Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal.

Authors: Cook S, de Almeida AP, de Matos AP, Esteves A, Lopes A, Parreira R, Piedade J
Journal: Virus Research
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Genetic+characterization+of+an+insect-specific+flavivirus+isolated+from+Culex+theileri+mosquitoes+collected+in+southern+Portugal

We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs.
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Genetic lineage characterization and spatiotemporal dynamics of classical insect-specific flaviviruses: outcomes and limitations

A diverse assemblage of RNA and DNA viruses found in mosquitoes collected in southern Portugal

Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal.

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