Detection of Theileria and Babesia infections amongst asymptomatic cattle in Portugal

Authors: Amaro A, Botelho A, Gomes J, Inácio J, Santos M, Santos-Gomes G, Soares R
Journal: Ticks and Tick-Borne Diseases
Link: http://www.ncbi.nlm.nih.gov/pubmed/23238244

Piroplasmosis caused by tick-borne hemoprotozoans of several Theileria and Babesia species has a major impact on livestock production worldwide. A reverse line blotting assay that includes genus- and species-specific probes for Theileria and Babesia species was used to assess the occurrence of these parasites in blood samples collected from 1407 healthy bovines throughout mainland Portugal.
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Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

Authors: Ahmed A, Amaro A, Costa P, Ferreira AS, Harstkeerl RA, Inácio J, Rocha T, Thompson G, Vieira ML
Publication Year: 2014
Journal: PLoS One
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112312

In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira.
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Rapid identification of veterinary-relevant Mycobacterium tuberculosis complex species using 16S rDNA, IS6110 and Regions of Difference-targeted dual-labelled hydrolysis probes

Authors: Albuquerque T, Amaro A, Botelho A, Costa P, Couto I, Ferreira AS, Inácio J, Machado D, Viveiros M
Publication Year: 2014
Journal: Journal of Virological Methods
Link: http://www.ncbi.nlm.nih.gov/pubmed/25192844

In this work we describe a rapid and robust two-step five-target probe-based real-time PCR identification algorithm, based on genomic deletion analysis, to identify the MTC species most commonly associated with TB in livestock and other animals.
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Detection of Theileria and Babesia infections amongst asymptomatic cattle in Portugal

Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

Rapid identification of veterinary-relevant Mycobacterium tuberculosis complex species using 16S rDNA, IS6110 and Regions of Difference-targeted dual-labelled hydrolysis probes

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