Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach
- Autores: Ahmed A, Amaro A, Costa P, Ferreira AS, Harstkeerl RA, Inácio J, Rocha T, Thompson G, Vieira ML
- Ano de Publicação: 2014
- Journal: PLoS One
- Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112312
In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira.