First isolation of human Leptospira strains, Azores, Portugal

OBJECTIVES: The aim of this study was the first identification of Leptospira isolates from Azorean inpatients. METHODS: Whole blood samples from 68 inpatients attending the São Miguel Hospital between 2006 and 2008, with a clinical and epidemiological suspicion of leptospirosis, were inoculated in a transport medium broth at the patient’s bedside and further processed using […]
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Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira.
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First isolation of human Leptospira strains, Azores, Portugal

Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

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