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Home / Archives for Silva MS

Immunoproteomic analysis of Plasmodium falciparum antigens using sera from patients with clinical history of imported malaria

  • Authors: Costa RM, De Sousa KP, Nogueira F, Silva MS, Vitorino R
  • Publication Year: 2013
  • Journal: Malaria Journal
  • Link: http://www.malariajournal.com/content/12/1/100

The malaria caused by Plasmodium falciparum remains a serious public health problem in the world, due largely to the absence of an effective vaccine. There is a lack of information on the structural properties and antigens capable of activating the immunological mechanisms for the induction of protective immunity. Therefore, the objective of this study is to evaluate the serological reactivity of sera from individuals with imported malaria and identify major immunogenic proteins.
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Prevalence and level of antibodies anti-plasmodium spp. in travellers with clinical history of imported malaria

  • Authors: Atouguia J, Costa RM, De Sousa KP, Silva MS, Tavira L
  • Publication Year: 2013
  • Journal: Journal of Parasitology Research
  • Link: http://www.hindawi.com/journals/jpr/2013/247273/

In this study, we show that 40.29% of travellers with a possible history of malaria exposure were positive for anti-Plasmodium spp. antibodies, while these individuals were negative by microscopy. The antibody test described here is useful to elucidate malaria exposure in microscopy-negative travellers from endemic countries.
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Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.

  • Authors: Atouguia J, De Sousa KP, Lança AS, Monteiro GA, Prazeres DM, Silva MS
  • Journal: Experimental Parasitology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Trypanosoma+brucei%3A+Immunisation+with+plasmid+DNA+encoding+invariant+surface+glycoprotein+gene+is+able+to+induce+partial+protection+in+experimental+African+trypanosomiasis.

Trypanosoma brucei is the etiological agent responsible for African trypanosomiasis, an infectious pathology which represents a serious problem of public health and economic losses in Sub-Saharan Africa.
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Molecular Tech- niques for Dicistrovirus Detection without RNA Extraction or Purification

  • Authors: Agirre J, Guérin DMA, Marti GA, Querido JFB, Silva MS
  • Journal: BioMed Research International
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/23710438

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
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Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

  • Authors: Agirre J, Guérin DMA, Marti GA, Querido JFB, Silva MS
  • Journal: Parasites & Vectors
  • Link: http://www.parasitesandvectors.com/content/6/1/66

Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family.
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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