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Home / Archives for Guérin DMA

Molecular Tech- niques for Dicistrovirus Detection without RNA Extraction or Purification

  • Authors: Agirre J, Guérin DMA, Marti GA, Querido JFB, Silva MS
  • Journal: BioMed Research International
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/23710438

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
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Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

  • Authors: Agirre J, Guérin DMA, Marti GA, Querido JFB, Silva MS
  • Journal: Parasites & Vectors
  • Link: http://www.parasitesandvectors.com/content/6/1/66

Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family.
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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