Molecular Tech- niques for Dicistrovirus Detection without RNA Extraction or Purification
- Autores: Agirre J, Guérin DMA, Marti GA, Querido JFB, Silva MS
- Journal: BioMed Research International
- Link: http://www.ncbi.nlm.nih.gov/pubmed/23710438
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.