Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection/quantification of different genospecies of Borrelia burgdorferi sensu lato

Authors: Nunes M, Parreira R, Carreira T, Inácio J, Vieira ML
Publication Year: 2017
Journal: Ticks and Tick-Borne Diseases
Link: https://www.sciencedirect.com/science/article/pii/S1877959X16302771

Nowadays, at least four clinically important B. burgdorferi sensu lato (s.l.) genospecies (B. afzelii, B. garinii, B. burgdorferi sensu stricto (s.s.) and B. lusitaniae) circulate in Portugal. Each genospecies has a different tropism that resuls in a diverse array of clinical manifestations. The standard diagnostic procedure used is normally simple, nevertheless, during the “window-period” phase, […]
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Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal

Authors: Campino L, Cotão AJ, Esteves A, Figueira L, Maia C, Nunes M, Parreira R, Pereira A, Vieira ML
Publication Year: 2017
Journal: Ticks and Tick-Borne Diseases
Link: https://www.sciencedirect.com/science/article/pii/S1877959X16301625

In the last decade, various research groups have reported a large diversity of new tick-borne phleboviruses, mostly prompted by the discovery of important human pathogens such as the Heartland and severe fever with thrombocytopenia syndrome viruses. Since these analyses have rarely been conducted using ticks collected from Southern Europe, this study was carried out so […]
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Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal

Authors: Campino L, Casadinho A, Maia C, Nunes M, Parreira R, Pereira A, Vieira ML
Publication Year: 2016
Journal: Parasites & Vectors
Link: https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1535-0

Tick-borne bacteria and protozoa by molecular sequencing The authors assess the presence of tick-borne bacteria and protozoa by molecular sequencing. Cervids and wild boars can can act as reservoir of different tick-borne pathogens, such as bacteria, parasites and viruses. The researchers collected 141 blood samples from free-ranging ungulates including 73 red deer, 65 wild boars and three fallow deer. Moreover, […]
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Molecular identification of Borrelia genus in questing hard ticks from Portugal: Phylogenetic characterization of two novel Relapsing Fever-like Borrelia sp.

Authors: Fingerle V, Lopes N, Maia C, Nunes M, Parreira R, Vieira ML
Publication Year: 2016
Journal: Infection Genetics and Evolution
Link: https://www.ncbi.nlm.nih.gov/pubmed/26976475

Molecular identification of Borrelia genus Several studies reported pathogenic species of Borrelia related to those that cause Tick-borne Relapsing Fever (RF). However, this studies unexpectedly suggested their transmission by hard ticks rather than by soft ticks. This study aimed to update the presence of B. burgdorferi s.l. species in ticks from several districts of Portugal, […]
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In vitro drug susceptibility of Leishmania infantum isolated from humans and dogs

Authors: Campino L, Henriques S, Maia C, Marques M, Nunes M, Rolão N
Publication Year: 2013
Journal: Experimental Parasitology
Link: http://www.ncbi.nlm.nih.gov/pubmed/23747751

Visceral leishmaniasis (VL) caused by parasites of Leishmania donovani complex is a severe human disease which often leads to death if left untreated. Domestic dogs are the main reservoir hosts for zoonotic human visceral infection caused by Leishmania infantum. In the absence of effective human and dog vaccines, the only feasible way to treat and control leishmaniasis is through the use of suitable medications.
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Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection/quantification of different genospecies of Borrelia burgdorferi sensu lato

Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal

Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal

Molecular identification of Borrelia genus in questing hard ticks from Portugal: Phylogenetic characterization of two novel Relapsing Fever-like Borrelia sp.

In vitro drug susceptibility of Leishmania infantum isolated from humans and dogs

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