Negeviruses found in multiple species of mosquitoes from southern Portugal: Isolation, genetic diversity, and replication in insect cell culture

• Authors: Abecasis A, Carapeta S, de Almeida AP, de Matos AP, do Bem B, Esteves A, Lopes A, McGuinness J, Parreira R, Piedade J
• Publication Year: 2015
• Journal: Virology
• Link: https://www.sciencedirect.com/science/article/pii/S0042682215002329%20

In this report, an RT-PCR approach based on the use of degenerate primers allowed the identification of negeviruses in four different species of mosquitoes (Ochlerotatus caspius, Culex pipiens, Cx. theileri and Cx. univittatus) collected in southern Portugal. The genomes of two of these viruses, sequenced to full completion, were shown to encode all the proteins […]
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