Molecular detection of bacterial and parasitic pathogens in hard ticks from Portugal

Autores: Campino L, Cardoso L, Ferreira A, Maia C, Nunes M, Vieira ML
Ano de Publicação: 2014
Journal: Ticks and Tick-Borne Diseases
Link: http://www.ncbi.nlm.nih.gov/pubmed/24745731

Ticks are important vector arthropods of human and animal pathogens. As information about agents of disease circulating in vectors in Portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. A total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of Portugal.
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Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

Autores: Ahmed A, Amaro A, Costa P, Ferreira AS, Harstkeerl RA, Inácio J, Rocha T, Thompson G, Vieira ML
Ano de Publicação: 2014
Journal: PLoS One
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112312

In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira.
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Human Leptospirosis: Seroreactivity and Genetic Susceptibility in the Population of Sao Miguel Island (Azores, Portugal)

Autores: Branco CC, Bulhões SM, Cabral R, Esteves LM, Mota FM, Mota-Vieira ML, Paiva C, Vieira ML
Ano de Publicação: 2014
Journal: PLoS One
Link: http://www.ncbi.nlm.nih.gov/pubmed/25255143

We conducted, in 2011, a retrospective hospital-based case-control study in the São Miguel Island population (Azores archipelago). In order to determine the seropositivity against pathogenic Leptospira after the first episode of leptospirosis, we performed a serological evaluation in 97 unrelated participants diagnosed with leptospirosis between 1992 and 2011.
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Molecular detection of bacterial and parasitic pathogens in hard ticks from Portugal

Direct detection and differentiation of pathogenic Leptospira species using a multi gene targeted real time PCR approach

Human Leptospirosis: Seroreactivity and Genetic Susceptibility in the Population of Sao Miguel Island (Azores, Portugal)

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