Early transcriptional response to chloroquine of the Plasmodium falciparum antioxidant defence in sensitive and resistant strains

• Authors: Bautista JM, Diez A, Do Rosário VE, Nogueira F, Pérez-Benavente S, Puyet A, Radfar A
• Journal: Acta Tropica
• Link: http://www.pubpdf.com/pub/20138820/Early-transcriptional-response-to-chloroquine-of-the-Plasmodium-falciparum-antioxidant-defence-in-se

Resistance to chloroquine (CQ) in Plasmodium falciparum has a major impact on malaria control worldwide. To gain insight into early parasite stress response, mRNA expression profiles were determined for a set of 10 antioxidant defence genes in synchronized CQ-sensitive (3D7) and CQ-resistant (Dd2) clones under transient IC50 CQ-exposure (Dd2, 200 nM; 3D7, 14 nM). Upon 2-h CQ challenge, the mRNA upregulation detected was greater in 3D7 (six genes overexpressed at 1/3 of the intraerythrocytic cycle) than in Dd2 clone (three genes responding), providing evidence of an early transcriptional response to CQ-induced oxidative stress which might underlie some of the parasite’s metabolic adaptation to the drug.
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