Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil

Authors: Clark TG, da Silva PA, Dalla Costa ER, Esteves LS, Gomes HM, Gomes LL, McNerney R, Pain A, Perdigão J, Portugal I, Rastogi N, Rossetti ML, Suffys PN, Unis G, Vasconcelos SE, Viveiros M
Publication Year: 2015
Link: http://jcm.asm.org/content/53/12/3805.full%20

We recently detected the spoligotype patterns of strains of Mycobacterium pinnipedii, a species of the Mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in Porto Alegre, South Brazil. Because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed […]
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Interplay between Mutations and Efflux in Drug Resistant Clinical Isolates of Mycobacterium tuberculosis

Authors: Coelho TS, Couto I, Machado D, Maschmann RA, Perdigão J, Pereira C, Portugal I, Ramos DF, Rossetti MLR, Silva PA, Viveiros M, von Groll A
Publication Year: 2017
Journal: Frontiers in Microbiology
Link: https://www.frontiersin.org/articles/10.3389/fmicb.2017.00711/full

Numerous studies show efflux as a universal bacterial mechanism contributing to antibiotic resistance and also that the activity of the antibiotics subject to efflux can be enhanced by the combined use of efflux inhibitors. Nevertheless, the contribution of efflux to the overall drug resistance levels of clinical isolates of Mycobacterium tuberculosis is poorly understood and […]
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Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola

Authors: Clemente S, Couto I, Machado D, Masakidi P, Perdigão J, Portugal I, Ramos J, Silva C, Taveira N, Viveiros M
Publication Year: 2017
Journal: Scientific Reports
Link: https://www.nature.com/articles/srep42814

Tuberculosis (TB) poses a serious public health problem in Angola. No surveillance data on drug resistance is available and nothing is known regarding the genetic diversity and population structure of circulating Mycobacterium tuberculosis strains. Here, we have genotyped and evaluated drug susceptibility of 89 Mycobacterium tuberculosis clinical isolates from Luanda. Thirty-three different spoligotype profiles corresponding […]
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Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance

Authors: Andres S, Aung HL, Beckert P, Clark TG, Coll F, Cook GM, Devi KRU, Diel R, Khumalo D, Klaos K, Kohl TA, Köser CU, Krause KL, Kumar SS, Machado D, Maphalala G, Merker M, Moradigaravand D, Nair D, Nakatani Y, Niemann S, Opel-Reading HK, Parkhill J, Peacock SJ, Perdigão J, Portugal I, Schön T, Swaminathan S, Viveiros M
Publication Year: 2017
Journal: Antimicrobial Agents and Chemotherapy
Link: https://www.ncbi.nlm.nih.gov/pubmed/28971867

A screening of more than 1,500 drug-resistant strains of Mycobacterium tuberculosis revealed evolutionary patterns characteristic of positive selection for three alanine racemase (Alr) mutations. We investigated these mutations using molecular modeling, in vitro MIC testing, as well as direct measurements of enzymatic activity, which demonstrated that these mutations likely confer resistance to d-cycloserine.
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Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages

Authors: Alves A, Anthony RM, Bergval I, Bessa TB, Campino S, Clark TG, Coll F, Crampin AC, de Oliveira Sousa E, Dheda K, Gey van Pittius NC, Glynn JR, Grandjean L, Hasan R, Hasan Z, Hibberd ML, McNerney R, Miranda A, Moore D, Pain A, Panaiotov S, Perdigão J, Phelan JE, Portugal I, Sampson SL, Sheen P, Streicher EM, van Helden PD, Viveiros M, Warren R
Publication Year: 2016
Journal: BMC Genomics
Link: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2467-y

Background Approximately 10 % of the Mycobacterium tuberculosis genome is made up of two families of genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE and PPE families are typified by their highly conserved N-terminal domains that incorporate proline-glutamate (PE) and proline-proline-glutamate (PPE) signature motifs. They are hypothesised […]
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Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil

Interplay between Mutations and Efflux in Drug Resistant Clinical Isolates of Mycobacterium tuberculosis

Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola

Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance

Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages

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