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Home / Archives for Freitas FB

Geographic dispersal and genetic diversity of tick-borne phleboviruses (Phenuiviridae, Phlebovirus) as revealed by the analysis of L segment sequences

  • Authors: Pimentel V, Afonso R, Nunes M, Vieira ML, Bravo-Barriga D, Frontera E, Martinez M, Pereira A, Maia C, Paiva-Cardoso MDN, Freitas FB, Abecasis AB, Parreira R
  • Publication Year: 2019
  • Journal: Ticks and Tick-Borne Diseases
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/31078467

The large diversity of new tick-borne phleboviruses, and the negative impacts of the virulent viruses on human/animal health have led to a growing interest in their analysis. In this report, new insights are brought out into the diversity of putative phleboviruses circulating in Portugal (both the continental territory and the islands of São Miguel, in […]
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Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera Psychodidae) from southern Portugal

  • Authors: Afonso MO, Campino L, Cristóvão JM, Freitas FB, Maia C, Parreira R
  • Publication Year: 2015
  • Journal: Parasites & Vectors
  • Link: https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-0787-4%20

Zoonotic visceral leishmaniasis caused by Leishmania infantum which is transmitted by phlebotomine sand flies (Diptera, Psychodidae) is endemic in the Mediterranean basin. The main objectives of this study were to (i) detect Leishmania DNA and (ii) identify blood meal sources in wild caught female sand flies in the zoonotic leishmaniasis region of Algarve, Portugal/Southwestern Europe. Phlebotomine sand flies were […]
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Novel multiregion hybridization assay for the identification of the most prevalent genetic forms of the human immunodeficiency virus type 1 circulating in Portugal

  • Authors: Esteves A, Freitas FB, Parreira R, Piedade J
  • Publication Year: 2013
  • Journal: AIDS Research and Human Retroviruses
  • Link: http://online.liebertpub.com/doi/abs/10.1089/aid.2012.0025

The most efficient method for HIV-1 genetic characterization involves full-genome sequencing, but the associated costs, technical features, and low throughput preclude it from being routinely used for the analysis of large numbers of viral strains. Multiregion hybridization assays (MHA) represent an alternative for a consistent genetic analysis of large numbers of viral strains. Classically, MHA rely on the amplification by real-time PCR of several regions scattered along the HIV-1 genome, and on their characterization with clade-specific TaqMan probes (also known as hydrolysis probes).
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Mosquito surveys and West Nile virus screening in two different areas of southern Portugal, 2004-2007

  • Authors: Almeida AP, Alves R, Esteves A, Freitas FB, Novo MT, Rodrigues JC, Sousa CA
  • Journal: Vector-Borne and Zoonotic Diseases
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Mosquito+Surveys+and+West+Nile+Virus+Screening+in+two+Different+Areas+of+Southern+Portugal

Longitudinal mosquito surveys were carried out in southern Portugal from 2004 to 2007, in a wetland area (Comporta, District of Setúbal) and around the perimeter of a dam irrigation plant that created the largest artificial lake in Europe, 250 km(2) (Alqueva, Districts of Evora and Beja).
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Species Composition and WNV Screening of Mosquitoes from Lagoons in a Wetland Area of the Algarve, Portugal.

  • Authors: de Almeida AP, Esteves A, Freitas FB, Novo MT
  • Journal: Frontiers in Physiology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Species+Composition+and+WNV+Screening+of+Mosquitoes+from+Lagoons+in+a+Wetland+Area+of+the+Algarve%2C+Portugal

The aim of this study was to evaluate mosquito abundance, species diversity, larval and adult population dynamics in seven lagoons integrated in the wetland coastal system of the Algarve, Portugal, in the summer of 2007, as well as the screening of these for West Nile virus (WNV).
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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