Enhanced Detection of Tuberculous Mycobacteria in Animal Tissues Using a Semi-Nested Probe-Based Real-Time PCR

• Autores: Albuquerque T, Amaro A, Botelho A, Costa P, Couto I, Cunha MV, Ferreira AS, Inácio J, Viveiros M
• Journal: PLoS One
• Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081337

Bovine tuberculosis has been tackled for decades by costly eradication programs in most developed countries, involving the laboratory testing of tissue samples from allegedly infected animals for detection of Mycobacterium tuberculosis complex (MTC) members, namely Mycobacterium bovis. Definitive diagnosis is usually achieved by bacteriological culture, which may take up to 6–12 weeks, during which the suspect animal carcass and herd are under sanitary arrest.
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