A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations.
- Autores: Alves S, Basto AP, Cornelis P, Leitão A, Martins C, Piedade J, Ramalho R, Soares H
- Journal: Journal of Biotechnology
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=A+new+cloning+system+based+on+the+OprI+lipoprotein+for+the+production+of+recombinant+bacterial+cell+wall-derived+immunogenic+formulations
The conjugation of antigens with ligands of pattern recognition receptors (PRR) is emerging as a promising strategy for the modulation of specific immunity. Here, we describe a new Escherichia coli system for the cloning and expression of heterologous antigens in fusion with the OprI lipoprotein, a TLR ligand from the Pseudomonas aeruginosa outer membrane (OM).