A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations.

Authors: Alves S, Basto AP, Cornelis P, Leitão A, Martins C, Piedade J, Ramalho R, Soares H
Journal: Journal of Biotechnology
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=A+new+cloning+system+based+on+the+OprI+lipoprotein+for+the+production+of+recombinant+bacterial+cell+wall-derived+immunogenic+formulations

The conjugation of antigens with ligands of pattern recognition receptors (PRR) is emerging as a promising strategy for the modulation of specific immunity. Here, we describe a new Escherichia coli system for the cloning and expression of heterologous antigens in fusion with the OprI lipoprotein, a TLR ligand from the Pseudomonas aeruginosa outer membrane (OM).
Read More

Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterization, expression and in silico modelling.

Authors: Cortes H, Hemphill A, Leitão A, Marcelino E, Martins TM, Morais JB, Nolasco S, Novo C
Journal: The Electronic Journal Information Systems Evaluation
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Besnoitia+besnoiti+protein+disulfide+isomerase+(BbPDI)%3A+molecular+characterization%2C+expression+and+in+silico+modelling

Besnoitia besnoiti is an apicomplexan parasite responsible for bovine besnoitiosis, a disease with a high prevalence in tropical and subtropical regions and re-emerging in Europe. Despite the great economical losses associated with besnoitiosis, this disease has been underestimated and poorly studied, and neither an effective therapy nor an efficacious vaccine is available.
Read More

A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations.

Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterization, expression and in silico modelling.

About GHTM

Contacts

Map