Performance of an In-House Human Immunodeficiency Virus Type 1 Genotyping System for Assessment of Drug Resistance in Cuba

Autores: Abrahantes Y, Aleman Y, Alvarez A, Fonseca C, Kourí V, Perez J, Perez L, Schrooten Y, Van Laethem K, Vandamme AM, Vinken L
Ano de Publicação: 2015
Journal: PLoS One
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0117176

As commercial human immunodeficiency virus type 1 drug resistance assays are expensive, they are not commonly used in resource-limited settings. Hence, a more affordable in-house procedure was set up taking into account the specific epidemiological and economic circumstances of Cuba. The performance characteristics of the in-house assay were evaluated using clinical samples with various subtypes […]
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Trends and predictors of transmitted drug resistance (TDR) and clusters with TDR in a local Belgian HIV-1 epidemic

Autores: Albert J, Balotta C, Boucher C, De Munter P, Derdelinckx I, Ferreira F, Gomez-Lopez A, Khouri R, Kostrikis LG, Kücherer C, Li G, Littsola K, Nielsen C, Paredes R, Pineda-Peña AC, Schrooten Y, Stanojevic M, Trovão NS, Van Laethem K, Van Ranst M, Van Wijngaerden E, Vandamme AM, Vercauteren J, Vinken L, Wensing A
Ano de Publicação: 2014
Journal: PLoS One
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0101738

We aimed to study epidemic trends and predictors for transmitted drug resistance (TDR) in our region, its clinical impact and its association with transmission clusters. We included 778 patients from the AIDS Reference Center in Leuven (Belgium) diagnosed from 1998 to 2012. Resistance testing was performed using population-based sequencing and TDR was estimated using the WHO-2009 surveillance list.
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Characterization of amino acids Arg, Ser and Thr at position 70 within HIV-1 reverse transcriptase

Autores: Bernatchez J, Camacho RJ, Covens K, De Wit S, Dekeersmaeker N, Götte M, Megens S, Theys K, Van Laethem K, Vandamme AM, Vinken L
Ano de Publicação: 2014
Journal: Acta Clinica Belgica
Link: http://www.ncbi.nlm.nih.gov/pubmed/25103592

The amino acid position 70 in HIV-1 reverse transcriptase (RT) plays an important role in nucleoside RT inhibitor (NRTI) resistance. K70R is part of the thymidine analog mutations, but also other amino acid changes have been associated with NRTI resistance, such as K70E and K70G. In this study, we investigated the in vivo selection of the HIV-1 RT mutations K70S and K70T and their in vitro effect on drug resistance and replication capacity.
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Performance of an In-House Human Immunodeficiency Virus Type 1 Genotyping System for Assessment of Drug Resistance in Cuba

Trends and predictors of transmitted drug resistance (TDR) and clusters with TDR in a local Belgian HIV-1 epidemic

Characterization of amino acids Arg, Ser and Thr at position 70 within HIV-1 reverse transcriptase

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