Generation and Characterization of Monoclonal Antibodies against Rift Valley Fever Virus Nucleoprotein
- Autores: Antunes S, Coetzer JAW, Domingos A, Esteves A, Fafetine JM, Neves L, Paweska JT, Rutten VPMG
- Ano de Publicação: 2013
- Journal: Transboundary and Emerging Diseases
- Link: http://www.ncbi.nlm.nih.gov/pubmed/24589098
Due to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source and from inactivated RVFV, in Western blot analysis and in an enzyme-linked immunosorbent assay (ELISA).