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Home / Publicações / Folate pathway modulation in Rhipicephalus ticks in response to infection

Folate pathway modulation in Rhipicephalus ticks in response to infection

  • Autores: Dias F, Couto J, Ferrolho J, Seron GS, Bell-Sakyi L, Antunes S, Domingos A
  • Ano de Publicação: 2019
  • Journal: Transboundary and Emerging Diseases
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/31231926

Folate pathways components were demonstrated to be present in RNA-sequencing data obtained from uninfected and pathogen-infected Rhipicephalus ticks. Here, PCR and qPCR allowed the identification of folate-related genes in Rhipicephalus spp. ticks and in the tick cell line IDE8. Genes coding for GTP cyclohydrolase I (gch-I), thymidylate synthase (ts) and 6-pyrovoyltetrahydropterin (ptps) were identified. Differential gene expression was evaluated by qPCR between uninfected and infected samples of four biological systems, showing significant upregulation and largest fold-change for the gch-I gene in the majority of the biological systems, supporting the selection for functional analysis by RNAi silencing. Efficient knockdown of the gch-I gene in uninfected and Ehrlichia canis-infected IDE8 cells showed no detectable impact on the capacity of the bacteria to invade or replicate in the tick cells. Overall, this work demonstrated an increase in the expression of some folate-related genes, though not always statistically significantly, in the presence of infection, suggesting gene expression modulation of these pathways, either as a tick response to an invader or manipulation of the tick cell machinery by the pathogens to their advantage. This discovery points to folate pathways as interesting targets for further studies.

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GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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