Ciclo de Sessões ‘Parcerias Justas e Equitativas’ “Parcerias Norte-Sul em Pesquisa: uma revisão dos aspetos de ética e de integridade” Lecturer: Fernando Cupertino | Professor de Saúde Coletiva na Faculdade de Medicina da UFG| Servidor aposentado da Secretaria de Estado de Saúde de Goiás| Assessor técnico do Conselho Nacional dos Secretários de Saúde -Conass| […]
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GHTM Sessions 2023: “Plasmodium vivax immunopathogenesis: The role of hematopoietic niches”
Lecturer: Fábio Costa | Institute of Biology, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas (UNICAMP), Brazil
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GHTM Sessions 2022: “Microbial Biotechnologies for Vector-Borne Diseases Control”
Lecturer: Etienne Bilgo| Institut de Recherche en Sciences de la Sante (IRSS), Burkina Faso
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GHTM Sessions 2022 – “Phlebotomine Sand Flies in Southern Thailand: Entomological Survey, Identification of Blood Meals and Molecular Detection of Trypanosoma spp. and Leishmania spp.”
“Phlebotomine Sand Flies in Southern Thailand: Entomological Survey, Identification of Blood Meals and Molecular Detection of Trypanosoma spp. and Leishmania spp.” Lecturer: Theerakamol Pengsakul| Health and Environmental Research Center, Faculty of Environmental Management, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand
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GHTM Sessions 2022 – “Solid-phase isothermal primer elongation using ferrocene-labelled dNTPs for the electrochemical detection of single nucleotide polymorphisms”
- De: 18/11/2022
- Até: 18/11/2022
- Localização: Sala Fraga de Azevedo | 11:00 - 13:00
The aim of this work was to develop a generic platform for the electrochemical detection of single nucleotide polymorphisms (SNPs) via isothermal solid-phase primer elongation and exploiting ferrocene-labelled 2’-deoxyribonucleoside triphosphates (dNFcTPs). Two examples of the application of this platform will be described, the first using Klenow (exo-) DNA polymerase for the detection of a single SNP linked to resistance to the antibiotic rifampicin in Mycobacterium tuberculosis and the second using isothermal recombinase polymerase amplification to the direct multiplexed detection of SNPs associated with a predisposition to bone facture and osteoporosis.
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