A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

Authors: Calzetta M, Perugini E, Seixas G, Sousa CA, Guelbeogo WM, Sagnon N, Della Torre A, Pinto J, Pombi M, Mancini E
Publication Year: 2018
Journal: Medical and Veterinary Entomology
Link: https://www.ncbi.nlm.nih.gov/pubmed/29344968

Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium […]
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The study of arboviroses transmitted by vectors in IHMT

Authors: Parreira R, Sousa CA
Publication Year: 2015
Journal: Anais do Instituto de Higiene e Medicina Tropical
Link: http://ihmtweb.ihmt.unl.pt/PublicacoesFB/Anais/Anais2014/index.html#3/z%20

The study of vector-borne diseases has always been an area of interest of the Instituto do Higiene do Medicina Tropical (IHMT). After the eradication of malaria in Portugal, the activity of IHMT in this field of knowledge focused mainly on the study of these diseases in tropical and subtropical regions. However with the (re)emergence of […]
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Limited genomic divergence between intraspecific forms of Culex pipiens under different ecological pressures

Authors: Gomes B, Wilding CS, Weetman D, Sousa CA, Novo MT, Savage H, Almeida APG, Pinto J, Donnelly MJ
Publication Year: 2015
Journal: BMC Evolutionary Biology
Link: https://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-015-0477-z

Divergent selection can be a major driver of ecological speciation. In insects of medical importance, understanding the speciation process is both of academic interest and public health importance. In the West Nile virus vector Culex pipiens, intraspecific pipiens and molestus forms vary in ecological and physiological traits. Populations of each form appear to share recent common […]
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Microencapsulation of citronella oil for solar-activated controlled release as an insect repellent

Authors: Alves GM, Andrade L, Azenha C, Forte M, Marques J, Mendes A, Oliveira C, Parpot P, Pereira AI, Ribeiro AR, Sousa CA, Tavares CJ
Publication Year: 2016
Journal: Applied Materials
Link: https://www.sciencedirect.com/science/article/pii/S235294071630138X

This study investigates the functionalization of titanium dioxide nanoparticles on the surface of polymeric microcapsules as a mean to control the release of encapsulated citronella through solar radiation. This allows for the release of a mosquito repellent without human intervention, as the sunlight works as a release activator. The TiO2 nanoparticles were synthetized using a […]
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Climate change and mosquito-borne disease

Authors: Nazareth T, Seixas G, Sousa CA
Publication Year: 2016
Journal: Climate Change and Health
Link: https://link.springer.com/chapter/10.1007%2F978-3-319-24660-4_12

Ecology, development, behaviour, and survival of mosquitoes as well as the transmission dynamic of pathogens, strongly depend on climatic factors. Models have been developed to predict forthcoming mosquito-borne diseases scenarios based on estimations of future climate patterns. However, the complex interplay of climate variables with the mosquito-host-pathogen systems rend the overall effect of the climate […]
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A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

The study of arboviroses transmitted by vectors in IHMT

Limited genomic divergence between intraspecific forms of Culex pipiens under different ecological pressures

Microencapsulation of citronella oil for solar-activated controlled release as an insect repellent

Climate change and mosquito-borne disease

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