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Home / Archives for Mansinho K

Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 hepatitis C virus infected patients in a Portuguese cohort

  • Autores: Brandão R, Marcelino R, Gonçalves F, Diogo I, Carvalho A, Cabanas J, Costa I, Brogueira P, Ventura F, Miranda A, Mansinho K, Gomes P
  • Ano de Publicação: 2018
  • Journal: Viruses
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/29701642

This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present […]
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Tuberculosis extensively resistant: a present reality. Literature review because of a clinical case

  • Autores: Antunes I, Batista J, Falcão V, Machado D, Mansinho K, Manso M, Miranda AC, Moneti V, Morais S, Pereira K, Rijo J, Santos T, Silva J, Viveiros M
  • Ano de Publicação: 2017
  • Journal: Revista Portuguesa de Doenças Infecciosas

Decades after tuberculosis having acquired the status of curable disease, the emergence of Mycobacterium tuberculosis strains resistant to the most effective anti-tuberculosis drugs used in first-line treatment have placed this disease again as a global threat to public health. The epidemiology, pathogenesis, diagnosis and approach of drug-resistant tuberculosis in Portugal and in the world are […]
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Anaerobic Bacteria with Clinical Relevance: Taxonomic and Morphological Classification, Presence in Human Microbiota and Microbiological Diagnosis

  • Autores: Alves J, Gonçalves E, Mansinho K, Peres S
  • Ano de Publicação: 2017
  • Journal: Acta Médica Portuguesa
  • Link: https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/8098

The wide burden of anaerobic bacteria colonizing human body comprises about 90% of its total biomass. The biotic relationship between humans and its microbiota sets reciprocal benefits, albeit with pathogenic potencial for the human being in particular dysbiosis situations. Infections adjacent to or originating from the skin or mucous membranes of the intestinal, genitourinary and […]
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Human gastric hyperinfection by Anisakis simplex: A severe and unusual presentation and a brief review

  • Autores: Baptista-Fernandes T, Belo S, Castro I, Ferreira PM, Mansinho K, Paixao P, Pinto-Marques P, Rodrigues M, Roque L, Toscano C
  • Ano de Publicação: 2017
  • Journal: International Journal of Infectious Diseases
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/28882665

Anisakiasis is an emerging marine food-borne zoonosis resulting from the accidental ingestion of Anisakis larvae, through the consumption of raw or undercooked infected seafood products. The first case of human gastric hyperinfection by Anisakis simplex with an unusual and severe presentation, occurring in a Portuguese woman, is described in this article. Over 140 anisakid larvae […]
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Detection of Treponema pallidum Sp. Pallidum DNA in Cerebrospinal Fluid (CSF) by Two PCR Techniques

  • Autores: Aguas MJ, Araújo C, Batista T, Castro R, Mansinho K, Pereira FDLM
  • Ano de Publicação: 2016
  • Journal: Journal of Clinical Laboratory Analysis
  • Link: http://onlinelibrary.wiley.com/doi/10.1002/jcla.21913/epdf

BACKGROUND: Laboratory diagnosis of neurosyphilis is complicated especially when it is asymptomatic, no single laboratory test result being appropriate to diagnose central nervous system infectivity caused by Treponema pallidum. Our objective was to evaluate two polymerase chain reaction (PCR) techniques for the detection of T. pallidum DNA in the cerebrospinal fluid (CSF) of patients with […]
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About GHTM

GHTM is a R&D Center that brings together researchers from IHMT with a track record in Tropical Medicine and International/Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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1349-008 Lisboa
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+351 213 652 600
+351 213 632 105

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