Events 2022 – External02 » 1st Conference FMUL/FMUC: Infectious Diseases and Vaccines
- De: 09/04/2022
- Até: 09/04/2022
- Localização: https://janssenpt.webex.com/join/fmul
Global Health and Tropical Medicine
Traditional light-microscopy of stained smears may be replaced by fluorescent stains.
The auramine-O Acid-Fast-Stain (AFS) does not stain the cell-wall (mycolic acids), but is a classical DNA/RNA stain, whereby the cell wall only protects the microorganisms from decolorization by the acid. Confirmation of this new concept opens up the possibility to use this stain in different contexts: a) Auramine-O AFS allows the simple detection of Schistosome eggs; b) Auramine-O AFS allows detection of mycobacteria in histological samples; c) newer nucleic acid-stains may increase (broaden) the application of this stain.
Drones can be used to transport laboratory samples in remote areas, but limited evidence exists on whether that transport affects the samples’ results. Given its potential, the Ministry of Health and the National Institute of Health of Mozambique partnered with VillageReach NGO to generate such data and 300 respiratory samples were transported by two drones, departing from, and returning to the «INS TB Reference Laboratory» in Marracuene district. The samples’ analysis concluded that their quality had not changed by flight, being comparable to land transport.
GHTM Sessions 2022 CCI Diagnostics » Year Calendar » Sharing GHTM’s Cross Cutting Issues Diagnostics’ activities, knowledge and methodologies in this area.
Diagnosis of HIV infection is based on invasive methods such as blood collection. Since sputum is the main biological product for tuberculosis diagnosis, we aim to develop a non-invasive methodology for the simultaneous detection of Mycobacterium tuberculosis and HIV in sputum samples of individuals suspected of co-infection using superparamagnetic nanoparticles for capturing the target.