A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium […]
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Comparative analyses reveal discrepancies among results of commonly used methods for Anopheles gambiaemolecular form identification.

Authors: Calzetta M, Caputo B, Della Torre A, Mancini E, Petrarca V, Pinto J, Santolamazza F, Vicente JL
Journal: Malaria Journal
Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Comparative+analyses+reveal+discrepancies+among+results+of+commonly+used+methods+for+Anopheles+gambiae+molecular+form+identification.

BACKGROUND:
Anopheles gambiae M and S molecular forms, the major malaria vectors in the Afro-tropical region, are ongoing a process of ecological diversification and adaptive lineage splitting, which is affecting malaria transmission and vector control strategies in West Africa. These two incipient species are defined on the basis of single nucleotide differences in the IGS and ITS regions of multicopy rDNA located on the X-chromosome.
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A novel nested polymerase chain reaction assay targeting Plasmodium mitochondrial DNA in field-collected Anopheles mosquitoes

Comparative analyses reveal discrepancies among results of commonly used methods for Anopheles gambiaemolecular form identification.

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