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Home / Archives for Methods in Molecular Biology

Direct detection of Theileria annulata in bovine blood samples using standard and isothermal DNA amplification approaches

  • Authors: Gomes J, Inácio J
  • Publication Year: 2015
  • Journal: Methods in Molecular Biology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/25399096

Tropical theileriosis is a tick-borne disease responsible for important health problems in cattle, caused by the hemoprotozoan Theileria annulata. Traditionally, detection of Theileria pathogens in infected animals requires the microscopic examination of stained-blood smears and serological methods.
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Overview and challenges of molecular technologies in the veterinary microbiology laboratory

  • Authors: Cunha MV, Inácio J
  • Publication Year: 2015
  • Journal: Methods in Molecular Biology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/25399084

Terrestrial, aquatic, and aerial animals, either domestic or wild, humans, and plants all face similar health threats caused by infectious agents. Multifaceted anthropic pressure caused by an increasingly growing and resource-demanding human population has affected biodiversity at all scales, from the DNA molecule to the pathogen, to the ecosystem level, leading to species declines and extinctions and, also, to host-pathogen coevolution processes.
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Nested and Multiplex Real-Time PCR Using Dual-Labeled Probes: Detecting and Discriminating Mycobacterium tuberculosis Complex Members in Cultures and Animal Tissues

  • Authors: Costa P, Couto I, Inácio J, Viveiros M
  • Publication Year: 2015
  • Journal: Methods in Molecular Biology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/25399092

Members of the Mycobacterium tuberculosis complex (MTC) are causative agents of tuberculosis (TB) in both humans and animals. In the last two decades, the accumulating knowledge of the nucleotide sequences of several genes, and of the whole genomes, of MTC members has allowed the development of novel molecular assays able to detect and discriminate between these species.
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About GHTM

GHTM is a R&D Unit that brings together researchers with a track record in Tropical Medicine and International & Global Health. It aims at strengthening Portugal's role as a leading partner in the development and implementation of a global health research agenda. Our evidence-based interventions contribute to the promotion of equity in health and to improve the health of populations.

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