- Autores: Da Luz Martins M, Flores O, Inácio J, Lucas S, Meyer W, Spencer-Martins I
- Journal: Clinical Microbiology and Infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Differentiation+of+Cryptococcus+neoformans+varieties+and+Cryptococcus+gattii+using+CAP59-based+loop-mediated+isothermal+DNA+amplification.
Members of the Cryptococcus species complex (C. neoformans and C. gattii) are opportunistic pathogens responsible for frequently fatal cases of meningoencephalitis. These yeasts have been classified into five serotypes. Serotypes A and D are assigned to C. neoformans var. grubii and C. neoformans var. neoformans, respectively, Serotype AD strains are hybrids and serotype B and C strains are considered to belong to the related but distinct species C. gattii. Previous studies have identified ‘serotype-associated’ alleles of several genes in the Cryptococcus species complex. We developed a loop-mediated isothermal DNA amplification method using CAP59 allele-specific primers to identify the serotypes A, D and B/C of the Cryptococcus species complex.